plko1 neo plasmids Search Results


lentiviral plasmids  (Addgene inc)
94
Addgene inc lentiviral plasmids
Lentiviral Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko1+neo+plasmids/pm37735569-350-19-23?v=Addgene+inc
Average 94 stars, based on 1 article reviews
lentiviral plasmids - by Bioz Stars, 2026-08
94/100 stars
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plko 1 shsv40 neo  (Addgene inc)
92
Addgene inc plko 1 shsv40 neo
Plko 1 Shsv40 Neo, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko1+neo+plasmids/pmc08112228-1428-19-22?v=Addgene+inc
Average 92 stars, based on 1 article reviews
plko 1 shsv40 neo - by Bioz Stars, 2026-08
92/100 stars
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plko 1 sharid1a  (Addgene inc)
92
Addgene inc plko 1 sharid1a
a-b, Validation of ARID1A knockout in parental and ARID1A knockout RMG1 (a) and OVCA429 (b) cells. Immunoblots are representative of three independent experiments with similar results. c, Top 10 metabolic pathways enriched by ARID1A knockout in OVCA429 cells determined by metabolites set enrichment analysis (MSEA). d, Contribution of glutamine to oxygen consumption in the indicated OVCA429 cells expressing <t>shARID1A</t> or control analyzed by Seahorse. n= 5 independent experiments. e, Colony formation and quantification of parental and ARID1A knockout OVCA429 cells with or without glutamine deprivation for 12 days. n= 4 independent experiments. f-g, A fluorescence glucose analog 2-NBDG-based glucose uptake assayed by flow cytometry analysis for the indicated parental and ARID1A knockout RMG1 (f) or OVCA429 (g) cells. n= 4 independent experiments. h, Colony formation of parental and ARID1A knockout RMG1 cells cultured in the medium with indicated concentration of glucose for 12 days. Shown are representative images of four independent experiments. Error bars represent mean with s.d. in d, e, f and g. P values were calculated using two-tailed Student t-test in d, e and Fisher’s least significant difference test in c.
Plko 1 Sharid1a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko1+neo+plasmids/pmc08168620-337-14-13?v=Addgene+inc
Average 92 stars, based on 1 article reviews
plko 1 sharid1a - by Bioz Stars, 2026-08
92/100 stars
  Buy from Supplier

Image Search Results


a-b, Validation of ARID1A knockout in parental and ARID1A knockout RMG1 (a) and OVCA429 (b) cells. Immunoblots are representative of three independent experiments with similar results. c, Top 10 metabolic pathways enriched by ARID1A knockout in OVCA429 cells determined by metabolites set enrichment analysis (MSEA). d, Contribution of glutamine to oxygen consumption in the indicated OVCA429 cells expressing shARID1A or control analyzed by Seahorse. n= 5 independent experiments. e, Colony formation and quantification of parental and ARID1A knockout OVCA429 cells with or without glutamine deprivation for 12 days. n= 4 independent experiments. f-g, A fluorescence glucose analog 2-NBDG-based glucose uptake assayed by flow cytometry analysis for the indicated parental and ARID1A knockout RMG1 (f) or OVCA429 (g) cells. n= 4 independent experiments. h, Colony formation of parental and ARID1A knockout RMG1 cells cultured in the medium with indicated concentration of glucose for 12 days. Shown are representative images of four independent experiments. Error bars represent mean with s.d. in d, e, f and g. P values were calculated using two-tailed Student t-test in d, e and Fisher’s least significant difference test in c.

Journal: Nature cancer

Article Title: Targeting glutamine dependence through GLS1 inhibition suppresses ARID1A-inactivated clear cell ovarian carcinoma

doi: 10.1038/s43018-020-00160-x

Figure Lengend Snippet: a-b, Validation of ARID1A knockout in parental and ARID1A knockout RMG1 (a) and OVCA429 (b) cells. Immunoblots are representative of three independent experiments with similar results. c, Top 10 metabolic pathways enriched by ARID1A knockout in OVCA429 cells determined by metabolites set enrichment analysis (MSEA). d, Contribution of glutamine to oxygen consumption in the indicated OVCA429 cells expressing shARID1A or control analyzed by Seahorse. n= 5 independent experiments. e, Colony formation and quantification of parental and ARID1A knockout OVCA429 cells with or without glutamine deprivation for 12 days. n= 4 independent experiments. f-g, A fluorescence glucose analog 2-NBDG-based glucose uptake assayed by flow cytometry analysis for the indicated parental and ARID1A knockout RMG1 (f) or OVCA429 (g) cells. n= 4 independent experiments. h, Colony formation of parental and ARID1A knockout RMG1 cells cultured in the medium with indicated concentration of glucose for 12 days. Shown are representative images of four independent experiments. Error bars represent mean with s.d. in d, e, f and g. P values were calculated using two-tailed Student t-test in d, e and Fisher’s least significant difference test in c.

Article Snippet: Plasmids and lentivirus/retrovirus infection plentiCRISPR v2 (#52961) and pMXS-SLC1A3 (#72873) were obtained from Addgene. pLKO.1-shARID1A (TRCN0000059090) was purchased from Open Biosystems (#RHS3979-201776877). pLKO.1-GLS1-shRNA1 (TRCN0000051134), pLKO.1-GLS1-shRNA1 (TRCN0000051135), pLKO.1-SMARCB1-shRNA1 (TRCN0000039585), pLKO.1-SMARCB1-shRNA2 (TRCN0000039587), pLKO.1-SMARCA4-shRNA1 (TRCN0000015549) and pLKO.1-SMARCA4-shRNA2 (TRCN0000015552) were obtained from the Wistar Institute Molecular Screening and Protein Expression Facility.

Techniques: Knock-Out, Western Blot, Expressing, Fluorescence, Flow Cytometry, Cell Culture, Concentration Assay, Two Tailed Test